These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr . it seems that the increased 1 , 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n , while the decrease of this branch may prevent these processes 用特异性抗体结合westemblot结果发现，正义或反义gnt一vcdna的转染并不引起pkb 、 p44 / 42mapk和mek蛋白质表达的变化，而gntv一s / h ” 21细胞pkbt308 、 5473位点磷酸化和免疫沉淀pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化为指标的pkb的活性都较mock细胞增加， gntv一as / h7721细胞中这些指标的变化则相反。
Part i effects of gnt - v on cell proliferation , cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method , it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated , and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased . oppositely , these parameters were reduced in cells transfected with antisense cdna of gnt - v . these results suggested that cell proliferation and dna synthesis were modulated by gnt - v 第一部分gnt - v对h7721细胞生长、 egf敏感性和egf受体的影响用mtt方法发现转染正义gnt - vcdna的h7721细胞增殖速度加快，而且无论是~ 3h - tdr的总参入量或每个细胞的参入量均见增加，说明dna合成增强，而转染反义gnt - vcdna的h7721细胞则完全相反， dna合成和细胞增殖速度均见降低，这提示gnt - v可调节细胞的生长。
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using . triplix kit . a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction . the ss - dna was reversely transcripted from total rna . double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i , the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro . a high titer and high recombinant ratio of cdna library was constructed 构建恶性疟原虫海南株红内期cdna表达文库提取红内期恶性疟原虫海南株总rna ，直接以总rna为模板使用cdna文库构建试剂盒，首先反转录合成ss一dna ，再扩增合成ds一dna ( cdna ) ，对扩增产物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶试剂盒纯化、回收20obp以上的片断，经与载体连接再用蛋白包装物包装后形成未扩增文库，最后扩增完成恶性疟原虫海南株红内期cdna表达文库的构建。
Thioredoxins , an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc - , are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda . by its disulfide / dithiol interchange reaction , this protein can transmit the regulatory signals to seleted targets ( enzymes , transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis , dna synthesis , transcription , protein disulfide reduction , protein repair , filamentous phage assembly , cell apoptosis and seeds germinating and so on 该蛋白质中含有保守的- cp ( g ) pc -氨基酸活性基序，该基序中的两个半胱氨酸残基可通过巯基二硫键的转换实现其氧化还原状态的变化和电子氢的传递，对细胞中与氧化还原相关的多种生理过程的调节起重要作用。通过同许多酶类、蛋白类、细胞内活性因子相藕连， trx能对光合作用、 dna复制、基因转录、细胞凋亡和生长、噬菌体组装、蛋白质的还原和修复信号传导等生理过程产生影响和调节。
